Your patients are telling you something their labs can’t.

The Scientific Longevity Method™ measures how the brain, autonomic nervous system, metabolism and body respond to stress, recover and adapt — giving you objective data to guide personalized health and longevity programs and reassess change over time.

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20M+assessments collected
35,000patients followed for 5 years
20+countries using Biotekna®
30+Years of research and development

Normal labs. Symptomatic patient. Now what?

Traditional laboratory testing provides essential information about chemistry and disease markers. But it does not always show how effectively the body’s regulatory systems are responding, adapting and recovering. The Scientific Longevity Method™ adds another layer: objective assessment of autonomic regulation, brain physiology, body composition, metabolic patterns and recovery.

The Scientific Longevity System™ · Powered by Biotekna®

One Method. Three Steps. Five Areas of application

    Objective assessmentsData you can trust
    Practical applicationsTools for real care
    Better insightsKnowledge that guides
    Better outcomesResults that matter
    Capabilities

    One System. Multiple dimensions of physiology.

    Systemic coherence = alignment across PFC · ANS · HPA · metabolism · ECM

    Through Biotekna® Plus, HRV biofeedback can run during a RegMatEx or TomEEx session — top-down and bottom-up regulation at the same time, in one patient record.

    The Method

    One method. Three steps.

    Start with the level that fits your practice today and expand without rebuilding your clinical workflow.

    Step 1

    Longevity Mapping

    Establish the patient’s autonomic baseline, challenge the system, measure its response, guide regulation and then reassess recovery.

    • HRV, stress index and sympathovagal balance
    • Reactivity to orthostatic, cognitive and motor stimuli
    • Cardiorespiratory biofeedback at resonance frequency
    • At-home continuity with ANS Control
    1 · Baseline Current state
    42SDNN ms
    31RMSSD ms
    1450Total Power
    Moderate coherence
    PPG Stress Flow · live sessionIllustrative values

    Step 2

    Predictive Longevity

    Expand the autonomic assessment with prefrontal cortex oxygenation and full body-composition analysis, then integrate the findings into a broader view of health, function and future risk.

    • Cerebral blood flow and cognitive efficiency (HEG)
    • Fat mass, lean mass, hydration, phase angle (BIA-ACC)
    • Predictive Health Index — integrated health and risk modeling
    • Full reassessment at 30 days to show change over time

    Intervention Layer

    Electrodermal Interventions

    For practices that want to extend the Method from assessment and biofeedback into in-office electrodermal intervention, RegMatEx and TomEEx add adaptive microcurrent and electrodermal tools with pre- and post-session reassessment.

    • Stress regulation and neuromodulation support
    • Extracellular matrix and tissue health analysis
    • Pre- and post-intervention comparison in the same session

    Step 3

    The Scientific Longevity System™

    The complete system: measure the Predictive Health Index at the first visit, intervene globally with lifestyle and the electrodermal layer, then reassess the risk stratification to show how the risk of chronic disease has changed.

    • Baseline Predictive Health Index from PPG, HEG and BIA-ACC
    • Lifestyle intervention combined with RegMatEx and TomEEx
    • Reassessment of the actual risk of developing chronic disease
    • Risk stratification documented over time in one patient record
    complete systemASSESSINTERVENEREASSESS1AssessMeasure the Predictive HealthIndex at the first visit.2InterveneLifestyle intervention andelectrodermal intervention,together.3ReassessMeasure again the actual risk ofdeveloping chronic disease.

    Become a Scientific Longevity Practitioner.

    Choose the entry point that fits your practice today. Every level includes Biotekna® Plus software, IWI training and ongoing clinical support, and you can expand within the same platform over time.

    Step 1

    Longevity Mapping

    PPG Stress Flow · ANS Control

    Devices included in Longevity Mapping
    Reports generated
    • ANS assessment report with HRV, stress and recovery metrics
    Ideal for
    • Best for autonomic assessment, HRV, stress and recovery
    Free booklet — Longevity Mapping

    How to run the six-phase protocol and read the PPG report, with the ANS Control and Melcalin® home prescription for this step.

    Book a discovery call
    Step 2

    Predictive Longevity

    PPG Stress Flow · ANS Control · BIA-ACC · HEG

    Devices included in Predictive Longevity
    Reports generated
    • Predictive Health Index
    • Brain / HEG and body-composition assessment
    Ideal for
    • Best for longevity, functional medicine and advanced preventive programs
    Free booklet — Predictive Longevity

    How to read the Predictive Health Index and the BIA-ACC and HEG reports, with the Melcalin® protocol for this step.

    Book a discovery call
    Intervention Layer

    Electrodermal Interventions

    RegMatEx · TomEEx

    Devices included in Electrodermal Interventions
    Reports generated
    • Adaptive microcurrent and electrodermal assessment with pre- and post-session comparison
    Ideal for
    • Best for practices wanting an intervention layer
    Free booklet — Electrodermal Interventions

    How RegMatEx and TomEEx sessions are structured, from the first assessment through the intervention cycles and reassessment.

    Book a discovery call
    Complete system

    The Scientific Longevity System™

    PPG Stress Flow · ANS Control · BIA-ACC · HEG · RegMatEx · TomEEx

    Devices included in the Scientific Longevity System
    Reports generated
    • Complete Scientific Longevity report suite
    • Predictive Health Index
    Ideal for
    • Best for full-service longevity clinics, multi-practitioner clinics, and performance / recovery programs
    Free: The Scientific Longevity Handbook

    The practical introduction to the Method. The dedicated booklet for this step is in production — we send it to you the moment it is ready.

    Book a discovery call

    Flexible financing available.

    36-, 48- and 60-month options may be available through third-party financing partners, subject to approval.

    Built for clinical and business sustainability.

    We help practitioners understand implementation, patient pathways, financing and potential practice economics during the discovery process.

    Investment, financing options and practice economics are discussed during the discovery call. Financing is provided by third-party lenders and subject to credit approval. Section 179 may allow qualifying equipment to be deducted in the year it is placed in service — confirm eligibility with your tax advisor.

    Not sure where to start?

    Find the right entry point for your practice in 30 seconds.

    The science behind the Method.

    Research Library │ Clinical Validation │ Scientific Leadership │ Education

    Over thirty years of instrumentation

    For more than thirty years Biotekna® has developed non-invasive instrumentation for the objective assessment of autonomic, metabolic and bioelectrical regulation. Its technologies are used in over twenty countries and have generated a database of more than twenty million assessments, supporting peer-reviewed research in stress physiology, body composition and electrodermal medicine.

    Dr. Gianluca Bianco, MD — Chief Medical Officer
    Chief Medical Officer

    Dr. Gianluca Bianco, MD

    Medical doctor specializing in internal medicine, with advanced training in acupuncture, auricular neuromodulation, PNEI and posturology. His work focuses on stress, pain and the fascial system.

    He leads the Research Laboratory in Posturology and Neuromodulation (RELPON) at Sapienza University of Rome and teaches predictive diagnostics, functional medicine and scientific acupuncture.

    Dr. Francisco Cidral, ND, PhD — Chief Executive Officer
    Chief Executive Officer

    Dr. Francisco Cidral, ND, PhD

    Naturopathic doctor and neuroscientist with graduate training in neuroscience and a post-doctorate in health sciences, with fifteen years of experience as a professor and researcher in integrative health.

    Co-founder and associate professor at the Laboratory of Experimental Neuroscience in Brazil. His current work focuses on photobiomodulation, neurophysiology and technologies for physical and mental health.

    Built on data, not on claims.

    Biotekna® technologies have been in clinical use for more than three decades, with a research base across autonomic assessment, body composition science and predictive health modeling.

    20M+Assessments in the Biotekna® database
    35,000Patients followed for five years
    20+Countries using Biotekna®
    30+Years of research and development

    Extend the Method beyond the office.

    Assessment findings can support personalized home strategies including lifestyle, nutritional and practitioner-directed programs, helping patients continue their plan between visits.

    Melcalin® protocols matched to each step of the Method

    Melcalin® food supplements are not intended to diagnose, treat, cure or prevent any disease. They do not replace a varied, balanced diet and a healthy lifestyle. Use according to product labeling and applicable regulations.

    Resources for practitioners.

    Start with the free Scientific Longevity Handbook, then use the complete practitioner reference for deeper clinical implementation.

    Free · 23 pages

    The Scientific Longevity Handbook

    A practical framework — by Bianco & Cidral

    • The essential method in a concise, practical format
    • From fragmented symptoms to systemic coherence
    • The recovery window and the tier logic explained
    • The right starting point before the full edition

    Free — we email it straight to you. No call required.

    Professional reference · 121 pages

    The Scientific Longevity Method™ — The Manual

    A 121-page professional implementation manual for the Scientific Longevity Method™ — by Gianluca Bianco & Francisco Cidral

    • All seven chapters, end to end
    • Selected bibliographic references
    • The definitive practitioner reference
    $580

    Available through a short online meeting with our team

    Peer-reviewed publications

    Thirty-five peer-reviewed articles by Dario Boschiero. A complete bibliography of identified and verified journal articles; book chapters, congress abstracts, preprints and datasets are excluded.

    2026

    01
    Extracellular Water and Phase Angle, Markers of Heightened Inflammatory State, and Their Extrapolative Potential for Body Composition Outcomes in Adults

    Cvijetić S, Boschiero D, Shin H, Reilly AS, Noorani ST, Vasiljevic N, Ilich JZ.

    Metabolites. 2026 Jan 2;16(1):40. doi: 10.3390/metabo16010040.

    02
    Predictive health index: integrating body composition and heart rate variability metrics with artificial intelligence to predict chronic disease risk and specific chronic non-communicable diseases

    Boschiero D, Gallotta A, Ferrari F, Dragoumani K, Lambrou GI, Vlachakis D, Chrousos GP.

    Hormones (Athens). 2026;25(1):181-193. doi: 10.1007/s42000-025-00727-2. Epub 2025 Dec 8.

    2025

    03
    Bioelectrical impedance analysis of body composition in children and adolescents with type 1 diabetes: a prospective case-control study

    Giardinelli S, Lambertini AG, Lumaca A, Boschiero D, Cavallin F, Zucchini S, Malaventura C, Suppiej A.

    Eur J Pediatr. 2025 Aug 18;184(9):560. doi: 10.1007/s00431-025-06401-4.

    04
    Cardio-respiratory coherence: a solution for restoring psycho-physical well-being, resilience and optimal performance

    Boschiero D, Cuzzolin F, Marro M, Ferrari F, Beltrami G, Pecorelli S.

    Med Sport. 2025;78(2):114-122. doi: 10.1728/4594.46017.

    05
    The interplay between heart rate variability, inflammation, and lipid accumulation: Implications for cardiometabolic risk

    Wiley CR, Pourmand V, Stevens SK, Jarczok MN, Fischer JE, Boschiero D, Poggiogalle E, Koenig J, Thayer JF, Williams DP.

    Physiol Rep. 2025 Apr;13(8):e70313. doi: 10.14814/phy2.70313.

    2024

    06
    Dysautonomia in athletes

    Boschiero D, Beltrami G, Marro M, Ferrari F, Sacco A, Pecorelli S.

    Med Sport. 2024;77(3):307-325. doi: 10.23736/S0025-7826.24.04480-6.

    07
    Medically Unexplained Symptoms Are Linked to Chronic Inflammatory Diseases: Is There a Role for Frontal Cerebral Blood Oxygen Content?

    Straub RH, Boschiero D.

    Neuroimmunomodulation. 2024;31(1):40-50. doi: 10.1159/000536204. Epub 2024 Jan 12.

    08
    Prevalence of Osteosarcopenic Adiposity in Apparently Healthy Adults and Appraisal of Age, Sex, and Ethnic Differences

    Cvijetić S, Keser I, Boschiero D, Ilich JZ.

    J Pers Med. 2024 Jul 23;14(8):782. doi: 10.3390/jpm14080782.

    2023

    09
    Body fat and muscle in relation to heart rate variability in young-to-middle age men: a cross sectional study

    Cvijetic S, Macan J, Boschiero D, Ilich JZ.

    Ann Hum Biol. 2023 Feb;50(1):108-116. doi: 10.1080/03014460.2023.2180089.

    10
    Osteosarcopenic Adiposity and Nutritional Status in Older Nursing Home Residents during the COVID-19 Pandemic

    Cvijetić S, Keser I, Boschiero D, Ilich JZ.

    Nutrients. 2023 Jan 1;15(1):227. doi: 10.3390/nu15010227.

    2022

    11
    Circulating FGF21 vs. Stress Markers in Girls during Childhood and Adolescence, and in Their Caregivers: Intriguing Inter-Relations between Overweight/Obesity, Emotions, Behavior, and the Cared-Caregiver Relationship

    Christaki EV, Pervanidou P, Papassotiriou I, Mantzou A, Giannakakis G, Boschiero D, Chrousos GP.

    Children (Basel). 2022 Jun 2;9(6):821. doi: 10.3390/children9060821.

    12
    Diurnal Salivary Cortisol in Relation to Body Composition and Heart Rate Variability in Young Adults

    Cvijetic S, Keser I, Jurasović J, Orct T, Babić Ž, Boschiero D, Ilich JZ.

    Front Endocrinol (Lausanne). 2022 Mar 9;13:831831. doi: 10.3389/fendo.2022.831831. eCollection 2022.

    13
    Photoplethysmography (PPG)-determined heart rate variability (HRV) and extracellular water (ECW) in the evaluation of chronic stress and inflammation

    Chrousos GP, Papadopoulou-Marketou N, Bacopoulou F, Lucafò M, Gallotta A, Boschiero D.

    Hormones (Athens). 2022 Sep;21(3):383-390. doi: 10.1007/s42000-021-00341-y. Epub 2022 Jan 14.

    14
    Social skills and psychopathology are associated with autonomic function in children: a cross-sectional observational study

    Cainelli E, Vedovelli L, Bottigliengo D, Boschiero D, Suppiej A.

    Neural Regen Res. 2022 Apr;17(4):920-928. doi: 10.4103/1673-5374.322464.

    15
    Stress, Inflammation and Metabolic Biomarkers Are Associated with Body Composition Measures in Lean, Overweight, and Obese Children and Adolescents

    Christaki EV, Pervanidou P, Papassotiriou I, Bastaki D, Valavani E, Mantzou A, Giannakakis G, Boschiero D, Chrousos GP.

    Children (Basel). 2022 Feb 21;9(2):291. doi: 10.3390/children9020291.

    2021

    16
    Assessment of Body Composition and Dietary Intake in Nursing-Home Residents: Could Lessons Learned from the COVID-19 Pandemic Be Used to Prevent Future Casualties in Older Individuals?

    Keser I, Cvijetić S, Ilić A, Colić Barić I, Boschiero D, Ilich JZ.

    Nutrients. 2021 Apr 29;13(5):1510. doi: 10.3390/nu13051510.

    17
    Heart rate variability in late pregnancy: exploration of distinctive patterns in relation to maternal mental health

    Kimmel MC, Fransson E, Cunningham JL, Brann E, Grewen K, Boschiero D, Chrousos GP, Meltzer-Brody S, Skalkidou A.

    Transl Psychiatry. 2021 May 14;11(1):286. doi: 10.1038/s41398-021-01401-y.

    18
    Increased extracellular water measured by bioimpedance analysis and increased serum levels of atrial natriuretic peptide in polymyalgia rheumatica patients : Signs of volume overload

    Günther F, Ehrenstein B, Hartung W, Boschiero D, Fleck M, Straub RH.

    Z Rheumatol. 2021 Mar;80(2):140-148. doi: 10.1007/s00393-020-00845-9.

    19
    Pre-surgical heart-rate variability strongly predicts less post-operative pain in patients with epilepsy

    Caton L, Bolzon M, Boschiero D, Thayer JF, Gidron Y.

    J Psychosom Res. 2021 Jun;145:110421. doi: 10.1016/j.jpsychores.2021.110421. Epub 2021 Mar 23.

    2020

    20
    Abnormal heart rate variability at school age in survivors of neonatal hypoxic-ischemic encephalopathy managed with therapeutic hypothermia

    Suppiej A, Vedovelli L, Boschiero D, Bolzon M, Cainelli E.

    Eur J Paediatr Neurol. 2020 Nov;29:66-70. doi: 10.1016/j.ejpn.2020.08.004. Epub 2020 Aug 20.

    21
    Chronic Stress Contributes to Osteosarcopenic Adiposity via Inflammation and Immune Modulation: The Case for More Precise Nutritional Investigation

    Ilich JZ, Gilman JC, Cvijetic S, Boschiero D.

    Nutrients. 2020 Apr 2;12(4):989. doi: 10.3390/nu12040989.

    22
    Heart Rate Variability in the Perinatal Period: A Critical and Conceptual Review

    Chiera M, Cerritelli F, Casini A, Barsotti N, Boschiero D, Cavigioli F, Corti CG, Manzotti A.

    Front Neurosci. 2020 Sep 25;14:561186. doi: 10.3389/fnins.2020.561186. eCollection 2020.

    23
    Sexual Dimorphism of Heart Rate Variability in Adolescence: A Case-Control Study on Depression, Anxiety, Stress Levels, Body Composition, and Heart Rate Variability in Adolescents with Impaired Fasting Glucose

    Stefanaki C, Michos A, Latsios G, Tousoulis D, Peppa M, Zosi P, Boschiero D, Bacopoulou F.

    Int J Environ Res Public Health. 2020 Apr 14;17(8):2688. doi: 10.3390/ijerph17082688.

    2019

    24
    Clinical validation of a non-invasive electrodermal biofeedback device useful for reducing chronic perceived pain and systemic inflammation

    Chrousos GP, Boschiero D.

    Hormones (Athens). 2019 Jun;18(2):207-213. doi: 10.1007/s42000-019-00098-5. Epub 2019 Feb 25.

    25
    Differences in brown adipose tissue activity with sex and anthropometry as determined by thermal imaging

    Muros-Molina J, Vazquez Rocha LC, Boschiero D, Morris DE, Law J, Budge H, Symonds ME.

    EMS Obesity J. 2019;1(1):002.

    26
    Lean Women on Metformin and Oral Contraceptives for Polycystic Ovary Syndrome Demonstrate a Dehydrated Osteosarcopenic Phenotype: A Pilot Study

    Stefanaki C, Bacopoulou F, Kandaraki E, Boschiero D, Diamandi-Kandarakis E.

    Nutrients. 2019 Sep 2;11(9):2055. doi: 10.3390/nu11092055.

    27
    Osteosarcopenic Obesity: Current Knowledge, Revised Identification Criteria and Treatment Principles

    Kelly OJ, Gilman JC, Boschiero D, Ilich JZ.

    Nutrients. 2019 Mar 30;11(4):747. doi: 10.3390/nu11040747.

    2018

    28
    Chronic stress and body composition disorders: implications for health and disease

    Stefanaki C, Pervanidou P, Boschiero D, Chrousos GP.

    Hormones (Athens). 2018 Mar;17(1):33-43. doi: 10.1007/s42000-018-0023-7. Epub 2018 Apr 27.

    2017

    29
    Bioimpedance analysis vs. DEXA as a screening tool for osteosarcopenia in lean, overweight and obese Caucasian postmenopausal females

    Peppa M, Stefanaki C, Papaefstathiou A, Boschiero D, Dimitriadis G, Chrousos GP.

    Hormones (Athens). 2017 Apr;16(2):181-193. doi: 10.14310/horm.2002.1732.

    30
    Increased extracellular water measured by bioimpedance and by increased serum levels of atrial natriuretic peptide in RA patients-signs of volume overload

    Straub RH, Ehrenstein B, Günther F, Rauch L, Trendafilova N, Boschiero D, Grifka J, Fleck M.

    Clin Rheumatol. 2017 May;36(5):1041-1051. doi: 10.1007/s10067-016-3286-x. Epub 2016 Apr 26.

    31
    MDCT of the liver in obese patients: evaluation of a different method to optimize iodine dose

    Rengo M, Bellini D, Businaro R, Caruso D, Azzara G, De Santis D, Picchia S, Biondi T, Eid M, Boschiero D, Laghi A.

    Abdom Radiol (NY). 2017 Oct;42(10):2420-2427. doi: 10.1007/s00261-017-1156-x.

    2016

    32
    Healthy overweight/obese youth: early osteosarcopenic obesity features

    Stefanaki C, Peppa M, Boschiero D, Chrousos GP.

    Eur J Clin Invest. 2016 Sep;46(9):767-78. doi: 10.1111/eci.12659. Epub 2016 Aug 11.

    2015

    33
    Stress and inflammatory biomarkers and symptoms are associated with bioimpedance measures

    Tsigos C, Stefanaki C, Lambrou GI, Boschiero D, Chrousos GP.

    Eur J Clin Invest. 2015 Feb;45(2):126-134. doi: 10.1111/eci.12388.

    2013

    34
    A Simple Non-Invasive Screening to Prevent Obesity through Body Composition Analysis (BIA-ACC)

    Ippoliti F, Boschiero D.

    J Obes Weight Loss Ther. 2013;3:169. doi: 10.4172/2165-7904.1000169.

    2008

    35
    Clinical nutrition in sports medicine: correlation between nutritional protocol and performance increase and athletes well-being

    Lamberti V, Sanson A, Boschiero D.

    Med Sport. 2008;61(2):261-266.

    List updated 17 September 2026.

    Assess it. Restore it. Reassess it.

    Tell us about your practice and we’ll show you which level of the Scientific Longevity Method™ fits your clinical model, patient population and goals.

    30 minutes with our team. No obligation.

    biofunktion.com  |  BIOFUNKTION LLC · Windermere, FL  |  Powered by Biotekna®

    Why standard assessment

    misses the real picture

    Chronic stress doesn't announce itself in a blood test. It breaks the body's regulatory systems from the top down — brain, autonomic nervous system, metabolism. The Functional Longevity Method measures every step of that cascade.

    1.

    ORIGIN · NEURO-AUTONOMIC

    Brain & autonomic regulation

    Chronic load shifts the balance between sympathetic and parasympathetic tone. Heart-rate variability and vascular response reveal the imbalance before the patient feels it.

    2.

    PROPAGATION · METABOLIC

    Metabolism & body composition

    Sustained autonomic strain reshapes energy use, fluid distribution and lean mass. Bio-impedance maps the metabolic cost long before standard labs move.

    3.

    OUTCOME · PREDICTIVE

    Predictive Health Index & Biological Age

    Integrates autonomic, neurological, endocrine, metabolic, inflammatory and body composition biomarkers to predicte health risk, personalized interventions, and monitor functional longevity.

    Not sure which layer your practice needs to start with? Take the 30-second quiz below.

    Courses &

    certifications

    Structured training on the Functional Longevity Method™ — start with the free

    introduction, then go deeper with certification programs.

    FOUNDATIONS -FREE-

    Course title — coming soon

    Short description of the free introductory course.

    Content TBC with Gianluca

    Certification -PAID-

    Course title — coming soon

    Short description of the free introductory course.

    Content + price TBC

    ADVANCED -PAID-

    Course title — coming soon

    Short description of the free introductory course.

    Content + price TBC

    Course details, pricing and enrollment links to be finalized with Dr. Bianco.